Development of hybrid elution systems for efficient purification of stilbenoids using centrifugal partition chromatography coupled to mass spectrometry

Abstract The phytochemicalstudy of the root extract of the stilbenoid-rich Vitis riparia× Vitis berlandieri grapevine was carried out by centrifugal partition chromatography(CPC). For this reason, we developed a new elutionmode we named back-step, which allowed us to obtain cleaner fractions and a more efficient separation process when used in conjunction with a classical elutionapproach. Three hydroxystilbenes: ( E )-resveratrol, ( E )- ɛ - viniferinand ( E )- vitisin C, with greater than 90% purity were thus obtained through such process, with minimal sample handling and purification steps. Online coupling of CPC to ESI mass spectrometry was used for optimization of the separation parameters and to facilitate the characterization of the stilbenoids. This study details the first phytochemicalinvestigation of stilbenoidsfrom the hybrid species together with a new elutionmode able to widen the range of ARIZONA biphasic systems.