Adenine methylation and antimicrobial resistance
2017
Development of antimicrobial
resistance(AMR) continues to be a global concern. In order to minimise the impact of such it is imperative to gather as much data as possible on the mechanisms and key pathways involved. The aim of this project was to investigate the role of adenine methylation in the development of
resistanceto
triclosanand associated antibiotic
cross-resistance.
Triclosan
resistancewas induced in E. coli through repeated exposure to sub-inhibitory concentrations. Pacbio SMRT sequencing was used to identify methylated bases in both wild type and
resistancebacteria. Modified bases within genes were identified through genome annotation, and differentially methylated genes
collated. E. coli rapidly became
resistantto
triclosanand exhibited increased
resistanceto multiple antibiotics. There were no statistically significant differences in global methylation. A number of genes associated with a range of functions were seen to be differentially methylated between the wild type and
resistantcultures.
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