HIV-1 RNA testing of pooled dried blood spots is feasible to diagnose acute HIV infection in resource limited settings
2018
AbstractObjectives: Rapid human immunodeficiency virus (HIV) antibody tests, routinely used for diagnosis in adults and older children in resource-limited settings (RLS), do not detect early HIV infections prior to
seroconversionor when antibody levels are still low. Nucleic acid amplification to detect HIV-1 RNA is the most sensitive method for
acute HIV infectiondiagnosis, but is costly. We therefore investigated HIV- 1 RNA testing of pooled
dried blood spots(DBS) to diagnose acute HIV infection.Design: Laboratory-based investigation.Methods: DBS were collected from HIV-1
Voluntary Counsellingand
Testing(HVCT) clients who tested negative on the Advanced QualityTM HIV antibody rapid test. DBS samples from five participants were pooled and tested on the COBAS AmpliPrep/COBAS
TaqManHIV-1 (CAP/CTM) Test v2. Individual DBS were tested when pools tested positive (> 200 RNA copies/ml). Acute infection was confirmed by HIV viral load testing, two fourth-generation HIV serological assays, and Geenius™ HIV ...
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