Analysis on the effect of parental origin of translocation and predictors for obtaining an euploid embryo in balanced translocation carriers

Abstract Research question What's the effect of parental origin of translocation and predictors for obtaining an euploid embryo in pre-implantation genetic testing for chromosomal structural rearrangements (PGT-SR) for balanced translocation carriers? Design 179 PGT-SR cycles and 614 blastocysts from 123 couples carrying a balanced translocation were retrospectively analyzed. Next-generation sequencing (NGS) was performed after trophectoderm biopsy. Results There were no differences in controlled ovarian stimulation (COS) parameters or PGT-SR outcomes regarding the number of oocytes retrieved (11.95 ± 5.71 vs 11.82 ± 6.26), blastulation rate (0.42 ± 0.27 vs 0.45 ± 0.28), biopsy cancellation rate (11.7% vs 12.9%), the number of blastocysts for biopsy (3.70 ± 2.58 vs 4.04 ± 3.51), the component of euploid embryos (24.12% vs 25.42%), aneuploid embryos (58.84% vs 57.52%) and mosaic embryos (17.04% vs 17.06%) between female carriers and male partner carriers. In a multivariate logistic regression model, the number of blastocysts for biopsy (adjusted OR 1.752 (1.359-2.259), p Conclusions The parental origin of translocation does not significantly affect the PGT-SR outcomes for young balanced translocation carriers. For obtaining one euploid embryo, at least 3.5 blastocysts are required. Couples should be informed that the probability of obtaining one euploid embryo is low when less than 4 blastocysts are obtained in one PGT cycle.