Alterations in the gut microbiome of children with severe ulcerative colitis
2012
Background: Although the role of microbes in disease pathogenesis is well established, data describing the variability of the vast
microbiomein children diagnosed with ulcerative colitis (UC) are lacking. This study characterizes the gut
microbiomein hospitalized children with severe UC and determines the relationship between microbiota and response to steroid therapy. Methods: Fecal samples were collected from 26 healthy controls and 27 children hospitalized with severe UC as part of a prospective multicenter study. DNA extraction, polymerase chain reaction (PCR) amplification of bacterial 16S rRNA, and microarray hybridization were performed. Results were analyzed in GeneSpring GX 11.0 comparing healthy controls with children with UC, and steroid responsive (n = 17) with nonresponsive patients (n = 10). Results: Bacterial signal strength and distribution showed differences between UC and healthy controls (adjusted P < 0.05) for Phylum, Class, Order, Family, Genus, and Phylospecies levels with reduction in
Clostridiaand an increase in Gamma-proteobacteria. The number of microbial phylospecies was reduced in UC (266 ± 69) vs. controls (758 ± 3, P < 0.001), as was the Shannon Diversity Index (6.1 ± 0.23 vs. 6.49 ± 0.04, respectively; P < 0.0001). Steroid nonresponders harbored fewer phylospecies than responders (142 ± 49 vs. 338 ± 62, P = 0.013). Conclusions: Richness, evenness, and biodiversity of the gut
microbiomewere remarkably reduced in children with UC compared with healthy controls. Children who did not respond to steroids harbored a
microbiomethat was even less rich than steroid responders. This study is the first to characterize the gut
microbiomein a large cohort of pediatric patients with severe UC and describes changes in the gut
microbiomeas a potential prognostic feature. (Inflamm Bowel Dis 2012)
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