Enzymatically Cross‐Linked Gelatin/Chitosan Hydrogels: Tuning Gel Properties and Cellular Response
2014
This work investigates the effect of combining physical and chemical gelation processes in a biopolymer blend: chitosan and tilapia fish
gelatin. Chemical (C) gels are obtained by cross-linking with the microbial enzyme transglutaminase at 37 °C. Hybrid physical-co-chemical (PC) gels are cross-linked at 21 °C, below
gelatingelation temperature. These protocols provide two microenvironments for the gelation process: in C gels, both
gelatinand chitosan are present as single strands; in PC gels, cross-linking proceeds within a transient physical gel of
gelatin, filled by chitosan strands. The chitosan/
gelatinchemical networks generated in PC gels show a consistently higher
shear modulusthan pure C gels; they are also less turbid than their C gels counterparts, suggesting a more homogeneous network. Finally, chitosan enhances the gels'
shear modulusin all gels. Proliferation assays show that
MC3T3cells proliferate in these mixed, hybrid gels and better so on PC gels than in C mixed gels.
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