Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci

Stuart Orkin, Daniel Bauer and colleagues present DNA Striker, a computational tool to design variant-aware saturating-mutagenesisscreens with multiple CRISPR-associated nucleases. They apply their methodology to the HBS1L- MYB intergenic region, which is associated with red-blood-celltraits, and identify putative regulatory elements that control MYBexpression.