Comprehensive overview of murine epididymal mononuclear phagocytes and lymphocytes: Unexpected populations arise
2018
Abstract Despite increasing evidence that
epididymal
immune disorderscan lead to infertility, the cells and mechanisms underlying
epididymalimmunity remain poorly understood. In this study, we propose a rather exhaustive overview of innate and adaptive immune cells present in the murine caput and cauda
epididymis. Using flow cytometry and a wide set of markers, we screened the broadest panel of immune cells ever, in this organ. For the first time, we unequivocally quantified the innate populations of monocytes, macrophages, and dendritic cells subtypes. We also revealed the presence of B cells,
gamma delta T cells, and
double negativeT cells in the murine
epididymis. They were localized by immunofluorescence stainings, and appeared to be all present in the interstitium and epithelium along the organ, but with respective preferential regional distribution. Altogether, these findings provide new insights on the actors and potential mechanisms involved in the immune responses against genital tract ascending pathogens and in the setting and maintenance of tolerance toward the
sperm cells.
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