[Growth inhibition of transfection of p16 gene to lung adenocarcinoma cell lines Anip973 and AGZY83-a].

Objective To investigate the suppression effect of tumor suppressor gene p16 on lung adenocarcinoma cell lines. Methods p16 expression vector was transfected into high metastasis lung carcinoma cell line, Anip973, and low metastasis lung cancer cell line, AGZY83-a. The suppression effects of p16 gene on cell growth was detected by cell growth curve, cloning efficiency assay, flow cytometry analysis and TUNEL technique. Results Overexpression of p16 gene in Anip973 and AGZY83-a could only lead the cells to arrest at G1 phase, but had no effects on cell growth curves, colony for mation rate and apoptosis. Conclusion Overexpression of p16 in Anip973 and AGZY83-a can not suppress the growth of the tumor cells. DOI: 10.3779/j.issn.1009-3419.2001.06.14