Aurora B Inhibitor TAK-901 Synergizes with BCL-xL Inhibition by Inducing Active BAX in Cancer Cells

Background: Aurora B kinaseplays an essential role in chromosome segregationand cytokinesis, and is dysregulated in many cancer types, making it an attractive therapeutic target. TAK-901 is a potent aurora B inhibitor that showed efficacy in both in vitro and in vivo oncology models. Materials and Methods: We conducted a synthetic lethalsiRNA screening to identify the genes that, when silenced, can potentiate the cell growth-inhibitory effect of TAK-901. Results: B-cell lymphoma-extra large ( BCL-xL) depletion by siRNA or chemical inhibition synergized with TAK-901 in cancer cell lines. As a mechanism of synthetic lethality, active BCL2 associated X, apoptosis regulator (BAX) was induced by TAK-901. BCL-xLprotected cells from BAX-dependent apoptosis induction. Therefore, TAK-901 sensitizes cancer cells to BCL-xLinhibition. Conclusion: Polyploidcells induced by TAK-901 are vulnerable to BCL-xLinhibition. Our findings may have an impact on combination strategies with aurora B inhibitors in clinical studies.