Electrosprayed Hydroxyapatite on Polymer Nanofibers to Differentiate Mesenchymal Stem Cells to Osteogenesis

Abstract Electrosprayingof hydroxyapatite (HA) nanoparticles onto the surface of polymer nanofibersprovides a potentially novel substrate for the adhesion, proliferation and differentiation of mesenchymal stem cells (MSCs) into bone tissueregeneration. HA nanoparticles (4%) were electrosprayedon the surface of electrospun polycaprolactone(PCL) nanofibers(420 ± 15 nm) for bone tissueengineering. PCL/HA nanofiberswere comparatively characterized with PCL/Collagen (275 ± 56 nm) nanofibersby FT-IR analysis to confirm the presence of HA. Fabricated PCL/HA and PCL/Collagen nanofibersand TCP (control) were used for the differentiation of equine MSC into osteogenic lineages in the presence of DMEM/F12 medium supplemented with β-glycerophosphate, ascorbic acid and dexamethasone. Cell proliferation and differentiation into an osteogenic lineage was evaluated by MTS assay, SEM observation, ALP activity, ARS staining, quantification of mineral deposition and expression of osteocalcin. Proliferation of MSCs ...