In vitro amoebicidal and antioxidant activities of some Tunisian seaweeds

Abstract Free-living amoebae of genus Acanthamoebaare opportunistic pathogens widely distributed in the environment, and are the causative agents of several humans’ infections, such as Acanthamoeba keratitis, Granulomatous Amoebic Encephalitisand also disseminated infections. The existence of the cyst stage complicates Acanthamoebatherapy as it is highly resistant to antibiotics and physical agents. All these facts reinforced the necessity to find and develop an effective therapy against Acanthamoeba infections. In the present study, we are interested to several seaweeds species collected from the Tunisian coasts and belonging to the 3 phyla (brown, green and red algae). The aim was to quantify the Total Phenolic Compounds in different organic extract, to evaluate antioxidant capacity (DPPH and ABTS) and to study the antiprotozoalactivity against A. castellanii Neff. The parasites have been inhibited by all extracts with an IC 50 ranged from 52,3 ± 1.8 μg/mL for ethyl acetate extract, to 134,6 ± 0.7 μg/mL for the hexanic one for the various species studied.