Identification of human ELOVL5 enhancer regions controlled by SREBP
2015
Abstract Fatty acid
elongase5 (ELOVL5) is an enzyme involved in the synthesis of
polyunsaturated fatty acids.
Sterol Regulatory Element-binding Protein(SREBP)-1 activates ELOVL5 and increases
polyunsaturated fatty acidsynthesis, which in turn negatively affects SREBP-1 expression. Thus, ELOVL5 has been established as an SREBP-1 target gene and an important component of the negative feedback loop of de novo
lipogenesis. However, the human ELOVL5 promoter/enhancer has not been fully analyzed and the location of SREBP biding sites around the ELOVL5 gene has yet to be defined. Here we performed a detailed promoter/enhancer analysis of human ELOVL5 gene, and identified two new SREBP binding sites, one in the 10 kb upstream region and one in the exon 1. These two SRE motifs are conserved among mammals and the mechanism found in the present study by which SREBP activates ELOVL5 is considered to be common in mammals. Through these findings, we clarified the molecular mechanism how SREBP activates ELOVL5, an important regulator of de novo
lipogenesis.
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