Diamide Does Not Improve The Analysis Of IL1β And TNFα In Dithiothreitol-treated Sputum Supernatants

There is one report (Milone et. al. ERS 2012) that diamide can be used to oxidize disulfide bonds that were separated by dithiothreitol (DTT) treatment during the homogenization of sputum samples, a process that can affect the analysis of mediators in sputum supernatants. It was the aim of this study to test this method for two selected cytokines in sputum samples of healthy subjects induced before and after inhalation of LPS.