O-GlcNAcylation blood levels are increased in response to stress induced by cardiopulmonary bypass

Introduction Cardiopulmonary bypass (CPB) is a common procedure to maintain body perfusion during cardiac surgery. It is associated with a systemic inflammatory response, hemostasis and circulatory dysfunction that can lead to organ dysfunction and seldomly to death. O-GlcNAcylation is a post-translational modification modulated in response to stress in different acute and chronic situations. Objective Decipher if blood O-GlcNAc levels are modified during CPB induced stress. Method Vingt adult patients with CPB time > 60 minutes were included. Blood samples were collected on EDTA at (i) anesthesia induction (Ind), (ii) aortic declamping (AD) and (iii) 5 hours post-declamping (AD + 5). Whole blood and plasma were recovered from samples. O-GlcNAc levels in blood were evaluated by western blot while plasmatic biological markers were measured on automatic laboratory analysis system to correlate O-GlcNAc level with systemic response to CPB. Demographics and One-Way ANOVA analysis were performed with RStudio. Results CPB induced an early increase of plasmatic inflammatory marker IL-6 (Ind: 5.7 ± 3.2, AD: 120.0 ± 26.5 pg/mL, P Conclusion In this preliminary study, we highlighted that the inflammation and tissue stress consecutive to CPB are associated with an increase in blood O-GlcNAc levels 5 hours after CPB. Inclusion of more patients and correlations between O-GlcNAc level variation and demographics and/or patients’ outcome might reveal a new biomarker of risk after CPB.