Spatial and temporal scales matter when assessing the species and genetic diversity of springtails (Collembola) in Antarctica

Seven species of springtail(Collembola) are present in Victoria Land, Antarctica and all have now been sequenced at the DNA barcodingregion of the mitochondrial cytochrome c oxidase subunit Igene (COI). Here, we review these sequence data (n = 930) from the GenBank and Barcodeof Life Datasystems (BOLD) online databasesand provide additional, previously unpublished sequences (n = 392) to assess the geographic distribution of COI variants across all species. Four species (Kaylathalia klovstadi, Cryptopyguscisantarcticus, Friesea grisea and C. terranovus) are restricted to northern Victoria Land and three (Antarcticinella monoculata, Cryptopygusnivicolus and Gomphiocephalus hodgsoni) are found only in southern Victoria Land, the two biogeographic zones which are separated by the vicinity of the Drygalski Ice Tongue. We found highly divergent lineages within all seven species (range 1.7 – 14.7%) corresponding to different geographic locations. Levels of genetic divergencefor the southern Victoria Land species Gomphiocephalus hodgsoni, the most widespread species (~ 27,000 km2), ranged from 5.9% to 7.3% at sites located within 30 km, but separated by glaciers. We also found that the spatial patterns of genetic divergencediffered between species. For example, levels of divergence were much higher for Cryptopygusterranovus (> 10%) than for Friesea grisea ( 5%) populations and over 87% of the total genetic variation (based on AMOVA) on either side of a single, 16 km width glacier. Collectively, these data provide evidence for limited dispersal opportunities among populations of springtailsdue to geological and glaciologicalbarriers (e.g. glaciers and ice tongues). Some locations harboured highly genetically divergentpopulations and these areas are highlighted from a conservation perspective as well as avoiding human- mediated transportbetween sites. We conclude that species-specific spatial and temporal scales need to be considered when addressing ecological and physiological questions as well as conservation strategies for Antarctic Collembola.