High content image analysis reveals function of miR-124 upstream of Vimentin in regulating motor neuron mitochondria
2018
microRNAs (miRNAs) are critical for neuronal function and their dysregulation is repeatedly observed in neurodegenerative diseases. Here, we implemented high content image analysis for investigating the impact of several miRNAs in mouse primary
motor neurons. This survey directed our attention to the neuron-specific miR-124, which controls axonal morphology. By performing next generation sequencing analysis and molecular studies, we characterized novel roles for miR-124 in control of mitochondria localization and function. We further demonstrated that the
intermediate filament
Vimentinis a key target of miR-124 in this system. Our data establishes a new pathway for control of mitochondria function in
motor neurons, revealing the value of a neuron-specific miRNA gene as a mechanism for the re-shaping of otherwise ubiquitously-expressed
intermediate filamentnetwork, upstream of mitochondria activity and cellular metabolism.
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