Means of evaluation and protection from doxorubicin-induced cardiotoxicity and hepatotoxicity in rats

Objectives: This work is aimed on the study of doxorubicin cardiotoxicityand hepatotoxicity in rats and the evaluation of protective effect of trimetazidineadministrated concomitantly with doxorubicinfor 3 days. Materials and Methods: Male Wistar rats used were subjected to different types of treatment (3 days); A: Control, B: Doxorubicintreatment and C: Trimetazidineand doxorubicintreatment. After sacrifice, tissular distribution of doxorubicin, cardiac scintigraphy, histological examination of the myocardium, and evaluation of liver function were assessed. Results: Obtained results show that doxorubicinhas a high affinity to tissues especially the heart. It causes hepatotoxicity and cardiotoxicitymarked by a significant increase of aspartate aminotransaminase (AST) and alanine aminotransaminase (ALT) levels and drop of the left ventricular ejection fraction (EF LV ) by scintigraphy. Histological examination showed general alteration of myocardium structure. Concomitant administration of trimetazidineattenuates significantly the cardiotoxicityand hepatotoxity induced by doxorubicin. Conclusion: We have evaluated the protective effect of trimetazidineon an animal model of doxorubicin-induced cardiotoxicityand hepatotoxicity. The evaluation of these effects were assessed by several means; tissular distribution of doxorubicin, histological examination, assessment of liver function, and EF LV by scintigraphythat characterizes the originality of this study.