Negative pressure wound therapy affects circulating plasma microRNAs in patients with diabetic foot ulcerationJagiellonian University Medical College.

Abstract Aims Negative pressure wound therapy (NPWT) is commonly used in diabetic foot ulceration (DFU). The molecular mechanisms of NPWT action, particularly outside of the wound site, have not been described. We assessed NPWT’s effect on circulating miRNA expression levels in type 2 diabetes (T2DM) patients with DFU. Methods We examined 34 T2DM patients treated with either NPWT (n=24) or standard therapy (ST, n=10). The group assignment was based on clinical criteria and local practice. Next-generation sequencing-based microRNA expression was determined on the patient’s plasma collected before therapy and after 8 days. Results NPWT patients were similar to the ST group in terms of age, BMI, and HbA1c level; however, they differed by mean wound area (12.6cm2 vs. 1.1cm2 p=0.0005). First, we analyzed the change of miRNA after NPWT or ST and observed an upregulation of let-7f-2 only in the NPWT group. Then, we analyzed the differential expression between NPWT and ST groups, looking at possible wound size effects. We found 12 differentially expressed miRNAs in pre-treatment comparison, including let-7f-2, while in post-treatment analysis we identified 28 miRNAs. The pathway enrichment analysis suggests that identified miRNAs may be involved in wound healing, particularly through angiogenesis. Conclusion We found initial evidence that NPWT in T2DM patients with DFU affects miRNA expression in plasma. Additionally, some differences in plasma miRNA expression may be related to wound size.