Trophic Factor and FR167653 Supplementation During Cold Storage Rescue Chronic Renal Injury

Purpose: The use of organs from deceased after cardiac death and extended criteria donors grew in the last decade. These organs are more sensitive to ischemia- reperfusion injuryduring transplantation and current preservation protocols do not protect them adequately.Materials and Methods: In an autotransplanted, deceased after cardiac death donor pig kidney model we evaluated the benefits of supplementation with University of Wisconsin solution trophic factors and FR167653, an inhibitor of p38 mitogen-activated proteinkinase.Results: Supplemented solution improved renal recovery and limited ischemia- reperfusion injury, particularly when agents were used in conjunction. Long-term benefits were highlighted by decreased renal fibrosis, as determined by Picrosirius staining, and inflammation, as evaluated by renal cell infiltration. Mechanistic evaluation showed decreased expression of epithelial-to-mesenchymal transition markers, a process involved in renal fibrosis development. Tumor necrosis factor-α was ...