Carvacryl acetate, a derivative of carvacrol, reduces nociceptive and inflammatory response in mice

Abstract Aims The present study aimed to investigate the potential anti-inflammatoryand anti- nociceptiveeffects of carvacryl acetate, a derivative of carvacrol, in mice. Main methods The anti-inflammatoryactivity was evaluated using various phlogistic agents that induce paw edema, peritonitis model, myeloperoxidase(MPO) activity, pro and anti-inflammatorycytokine levels. Evaluation of antinociceptive activity was conducted through aceticacid-induced writhing, hot plate test, formalin test, capsaicinand glutamate tests, as well as evaluation of motor performance on rotarod test. Key findings Pretreatment of mice with carvacryl acetate(75 mg/kg) significantly reduced carrageenan-induced paw edema ( P 2 and compound 48/80. In the peritonitis model, carvacryl acetatesignificantly decreased total and differential leukocyte counts, and reduced levels of myeloperoxidaseand interleukin-1 beta (IL-1β) in the peritoneal exudate. The levels of IL-10, an anti-inflammatorycytokine, were enhanced by carvacryl acetate. Pretreatment with carvacryl acetatealso decreased the number of aceticacid-induced writhing, increased the latency time of the animals on the hot plateand decreased paw lickingtime in the formalin, capsaicinand glutamate tests. The pretreatment with naloxone did not reverse the carvacryl acetate-mediated nociceptiveeffect. Significance In conclusion, the current study demonstrated that carvacryl acetateexhibited anti-inflammatoryactivity in mice by reducing inflammatory mediators, neutrophil migration and cytokine concentration, and anti- nociceptiveactivity due to the involvement of capsaicinand glutamate pathways.