Efficiency of 2.45 and 5.80 GHz microwave irradiation for a hydrolysis reaction by thermostable β-Glucosidase HT1

Microwave irradiation at different frequencies gave unique results for the hydrolyses of glycosyl bonds by β-Glucosidase HT1. With the observed relative complex permittivity data for the reaction buffer, 2.45 GHz microwave radiation affected both waters and ions, while 5.80 GHz only affected waters. We, here, propose that would be one of the unique “microwave nonthermal effects”.