Early diagnostics of septicemia using microRNA flow cytometry

From 2000 to 2008 the incidence of sepsis in hospitals increased by 83%, but we still lack effective diagnostic methods. Recent advances in probe chemistry have enabled scientists to better detect RNA using flow cytometry. This technique could potentially be used for acute and efficient detection of sepsis. However, it still requires substantial validation before it reaches full potential in the clinic. We are in the process of creating a robust microRNA flow cytometry assay that can be used in the clinic for early detection of sepsis. In order to do this, first a reference control and diagnostic microRNA biomarkers, specific for detection of septicemia, must be identified and validated. Next, a microRNA flow cytometry assay will be developed and validated with the use of the reference control and diagnostic microRNAs both intracellularly and in serum. Lastly, the developed flow cytometry assay, including controls and diagnostic biomarkers will be verified against blood samples of clinical patients both positive and negative for sepsis. An effective sepsis diagnostic will save lives, aid in drug discovery, and decrease health care costs. This project provides a unique opportunity to create a robust flow cytometry assay to measure expression differences in microRNAs to detect septicemia in the clinic. We are hopeful that our continued efforts to validate and standardize previously published microRNA detection assays and expand the possible new targets will lead to quicker and more robust methods to survey for sepsis in clinics.