Centrifugal micro-channel array droplet generation for highly parallel digital PCR
2017
Stable water-in-oil emulsion is essential to digital PCR and many other bioanalytical reactions that employ droplets as
microreactors. We developed a novel technology to produce monodisperse emulsion droplets with high efficiency and high throughput using a bench-top centrifuge. Upon centrifugal spinning, the continuous aqueous phase is dispersed into monodisperse droplet jets in air through a micro-channel array (
MiCA) and then submerged into oil as a stable emulsion. We performed dPCR reactions with a
high dynamic rangethrough the
MiCAapproach, and demonstrated that this cost-effective method not only eliminates the usage of complex microfluidic devices and control systems, but also greatly suppresses the loss of materials and cross-contamination.
MiCA-enabled highly parallel emulsion generation combines both easiness and robustness of picoliter droplet production, and breaks the technical challenges by using conventional lab equipment and supplies.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
47
References
86
Citations
NaN
KQI