Systems biosynthesis of secondary metabolic pathways within the oral human microbiome member Streptococcus mutans
2015
Streptococcus mutans, a Gram-positive human commensal and pathogen, is commonly recognized as a primary causative agent in dental caries. Metabolic activity of this strain results in the creation of acids and secreted products are recognized as pathogenic factors and agents that promote immunomodulation by stimulating the release of pro-inflammatory cytokines. Products of
secondary metabolicpathways of microorganisms from the
human microbiomeare increasingly investigated for their immunomodulatory functions. In this study, we sought to explore the
metabolomicoutput of
nonribosomal peptidepathways within the model S. mutans strain, S. mutans UA159, using a systems
metabolomicapproach to gain in-depth analysis on products created by this organism and probe these molecules for their immunomodulatory function. Comparative
metabolomicsand biosynthetic studies using wild-type and
nonribosomal peptidedeletion strains (within the mutanobactin biosynthetic locus), precursor feedings (fatty acid derivatives) led to the identification of 58 metabolites, 13 of which were structurally elucidated. In addition to these, an assembly line
derailmentproduct, mutanamide, was also identified and used to assess immunomodulatory properties of mutanobactins and actions relating to their previously reported functions describing hyphal inhibitory profiles in Candida albicans. The results of this study demonstrate both the complexity and the divergent roles of products stemming from this unique biosynthetic assembly line.
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