Allelic variation at the rpv1 locus controls partial resistance to Plum pox virus infection in Arabidopsis thaliana
2015
Sharka is caused by Plum pox virus (PPV) in stone
fruit trees. In orchards, the virus is transmitted by aphids and by grafting. In
Arabidopsis, PPV is transferred by mechanical inoculation, by
biolisticsand by agroinoculation with infectious cDNA clones. Partial resistance to PPV has been observed in the Cvi-1 and Col-0
Arabidopsisaccessions and is characterized by a tendency to escape systemic infection. Indeed, only one third of the plants are infected following inoculation, in comparison with the susceptible Ler accession. Genetic analysis showed this partial resistance to be
monogenicor digenic depending on the allelic configuration and recessive. It is detected when inoculating mechanically but is overcome when using
biolisticor agroinoculation. A genome-wide association analysis was performed using multiparental lines and 147
Arabidopsisaccessions. It identified a major genomic region, rpv1. Fine mapping led to the positioning of rpv1 to a 200 kb interval on the long arm of chromosome 1. A candidate gene approach identified the chloroplast
phosphoglycerate kinase(cPGK2) as a potential gene underlying the resistance. A virus-induced gene silencing strategy was used to knock-down cPGK2 expression, resulting in drastically reduced PPV accumulation. These results indicate that rpv1 resistance to PPV carried by the Cvi-1 and Col-0 accessions is linked to allelic variations at the
ArabidopsiscPGK2 locus, leading to incomplete, compatible interaction with the virus.
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