Developmental pattern of GFAP and vimentin gene expression in rat brain and in radial glial cultures
1995
In the present study we analyze the events which occur during the early stages of astrogliogenesis by examining the pattern of both GFAP and
vimentingene expression and their corresponding immunoreactive proteins during rat brain development. This study was carried out “in vivo” (whole brain) and “in vitro” (primary culture of radial glia) using immunofluorescence, immunoblotting, and Northern blot analysis. Our results demonstrate that although GFAP immunostaining appeared late in gestation and at day 5 in radial glia cultures, GFAP mRNA expression was first detected, at very low levels, on fetal (F) day 15 and increased to F21. During postnatal development a striking increase in GFAP and its encoding messenger occurs. In contrast, the levels of
vimentinand its mRNA expression were very high during the fetal stage (F15 to F21). Thereafter
vimentinexpression declined during postnatal (P) development until P21 and then remained constant at adult levels. In contrast, an increase in
vimentinexpression was observed in glial cells throughout the entire culture period. The biological significance of the developmental patterns of GFAP and
vimentinexpression in astroglial cells during brain development is discussed. © 1995 Wiley-Liss, Inc.
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