P099 : Topical eupatilin as a PPARα activator is effective for atopic dermatitis

Background: We observed that eupatilin, an active flavone derived from Artemisia plantspecies, functions as a PPAR メ activator through in vitro study. Objectives: We evaluated whether sequential application of topical eupatilinafter topical glucocorticoid (GC) could be more effective than GC alone for AD lesion in ADmurine model. Also, we conducted clinical trials to assess the efficacy and safety of Artemisia argyiextracts containing eupatilinfor AD patients. Methods: In animal experiment, GC was applied on inflamed skin of hairlessmice only in the first day of the experiment, with 3 subsequent consecutive days of treatment with eupatilinas well as bezafibrateas a positive control, or vehicle as a negative control. The efficacy of eupatilinwas evaluated by gross feature and skin barrier function. For clinical study, thirty subjects were recruited and randomly applied with active cream (treated site) or vehicle cream (control site) to the left or right side of the whole body twice a day for 4 weeks. Treatment outcomes were assessed by EASI score, itchscore and skin barrier function. Results: In the animal study, topical eupatilinimproved eczema lesion and skin barrier function. In the clinical study, a significant decrease in the EASI and itchscores (p<0.05) was observed in the treated sites. The skin barrier functionwas also improved. Any remarkable side effects related with the active cream were not found. Conclusion: Topical application of eupatilincould improve AD lesions.