Experimental infection with Escherichia coli O149:F4ac in weaned piglets
2006
Abstract The outcome of experimental intestinal infections with
enterotoxigenic Escherichia coli( ETEC ) is dependent on several factors. An important factor is adhesion of the challenge strain to the intestinal mucosa. The test for
susceptibilitytowards ETEC adhesion has so far been made by an
intestinal adhesiontest made after slaughter of piglets. However, in an experimental infection study with the purpose to obtain diarrhoeic piglets, it would be an advantage to test for
susceptibilityprior to experimentation. The Mucin 4 gene on porcine
chromosome 13has been proposed as a candidate gene for the production of the specific ETEC F4ab/ac receptor, and a DNA marker-based test has been developed to allow genotyping for ETEC F4ab/ac resistance/
susceptibility[Jorgensen, C.B., Cirera, S., Archibald, A.L., Anderson, L., Fredholm, M., Edfors-Lilja, I., 2004. Porcine polymorphisms and methods for detecting them. International application published under the
patent cooperation treaty(PCT). PCT/DK2003/000807 or WO2004/048606-A2]. The aim of this study was to test an experimental model for ETEC O149:F4ac -induced diarrhoea in piglets, selected for
susceptibilitytowards ETEC O149:F4ac adhesion prior to experimentation using a DNA marker-based test. Sixty-two healthy 25–32 days old recently weaned Danish crossbred piglets were used. All piglets were tested prior to experimentation for
susceptibilityor resistance towards ETEC O149:F4ac adhesion. Thirty-nine piglets, both
susceptibleand resistant, were oro-gastric intubated with 10 9 CFU of ETEC O149:F4ac and 23 age-matched piglets, both
susceptibleand resistant, were used as non-infected controls. Of
susceptiblepiglets, challenged with ETEC O149:F4ac , 74% had ETEC O149:F4ac -associated diarrhoea first day after first challenge, which were significantly higher relatively to the resistant and challenged piglets where 20% had diarrhoea ( p = 0.04). This study suggests a model for experimental ETEC induced diarrhoea.
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