Metabolomics Reveals the Molecular Mechanisms of Copper Induced Cucumber Leaf (Cucumis sativus) Senescence
2018
Excess copper may disturb plant photosynthesis and induce leaf senescence. The underlying toxicity mechanism is not well understood. Here, 3-week-old cucumber plants were foliar exposed to different copper concentrations (10, 100, and 500 mg/L) for a final dose of 0.21, 2.1, and 10 mg/plant, using CuSO4 as the Cu ion source for 7 days, three times per day.
Metabolomicsquantified 149 primary and 79 secondary metabolites. A number of intermediates of the
tricarboxylic acid(TCA) cycle were significantly down-regulated 1.4–2.4 fold, indicating a perturbed
carbohydrate metabolism. Ascorbate and aldarate metabolism and shikimate-
phenylpropanoidbiosynthesis (antioxidant and defense related pathways) were perturbed by excess copper. These metabolic responses occur even at the lowest copper dose considered although no phenotype changes were observed at this dose. High copper dose resulted in a 2-fold increase in
phytol, a degradation product of chlorophyll. Polyphenol
metabolomicsrevealed that some flavonoids ...
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