Genetic analysis of LRRK2 in Parkinson's disease in Han Chinese population.

Abstract Mutations in Leucine-rich repeatkinase 2 ( LRRK2) are recognized as the most frequent genetic factors contributing to Parkinson's disease (PD). The aim of our study was to explore LRRK2variants in PD patients within the mainland Han Chinese population. The whole coding regionsof LRRK2from 296 PD patients were sequenced by targeted regions sequencing and exome sequencing. Eighteen rare variants were identified in 27 PD patients, and 13 of them (M100T, L153W, A459S, S722N, R792K, C925Y, R981K, S1007T, V1447M, R1677S, N2308D, N2313S, and S2350I) were firstly reported in PD. We also tried to explore the genotype-phenotype associations of LRRK2variants in our data and found that PD with common and rare LRRK2variants was more likely to have motor fluctuation and nonmotor symptoms. The identification of novel variants in LRRK2suggests that this gene plays an important role in the pathogenesis and phenotype of PD in Han Chinese population, and our data also rang the alarm bell-more attention should be paid to the whole coding regionsof LRRK2.