Cyprinid herpesvirus 3 Evolves In Vitro through an Assemblage of Haplotypes that Alternatively Become Dominant or Under-Represented
2019
Viruses are able to evolve in vitro by mutations after
serial passagesin cell cultures, which can lead to either a loss, or an increase, of virulence.
Cyprinid herpesvirus 3(CyHV-3), a 295-kb double-stranded
DNA virus, is the etiological agent of the
koi herpesvirusdisease (KHVD). To assess the influence of
serial passages, an isolate of CyHV-3 (KHV-T) was passaged 99 times onto
common carpbrain (CCB) cells, and virus virulence was evaluated during passages through the experimental infections of
common carp. After 78 CCB passages, the isolate was much less virulent than the original form. A
comparative genomicanalysis of these three forms of KHV-T (P0, P78 and P99) revealed a limited number of variations. The largest one was a deletion of 1363 bp in the predicted ORF150, which was detected in P78, but not in P99. This unexpected finding was confirmed by conventional PCR and digital PCR. The results presented here primarily suggest that, CyHV-3 evolves, at least in vitro, through an assemblage of haplotypes that alternatively become dominant or under-represented.
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