Cloning of a centromere binding factor 3d (CBF3D) gene from Candida glabrata.

The gene encoding centromerebinding factor 3d (CBF3D) of the human pathogenicyeast Candida glabratahas been isolated by hybridization of Saccharomyces cerevisiae CBF3D (ScCBF3D) DNA to a C. glabrata partial genomic library. Sequence analysis revealed a 540 bp open reading frameencoding a protein of 179 amino acids with a calculated molecular mass of 20·9 kDa. The amino acid sequence is highly homologous (78·6% identity) to ScCbf3d and 48·3% identical to the human homologue p19 ( SKP1). Southern blotanalysis indicates that CgCbf3d is encoded by an unique nuclear gene. The cloned CgCBF3D gene can functionally substitute the S. cerevisiae homologue in a S. cerevisiae CBF3D-deletion mutant. The GenBankAccession No. for this gene is AF 072472. Copyright © 1999 John Wiley & Sons, Ltd.