Anti-miRNA21 and resveratrol-loaded polysaccharide-based mesoporous silica nanoparticle for synergistic activity in gastric carcinoma

AbstractAnti-miR21 and resveratrol (RSV)-loaded mesoporous silica nanoparticles conjugated with hyaluronic acid (HA) were developed to enhance therapeutic efficacy in gastric carcinoma. The surface conjugation of HA, which acts as a targeting ligand to the overexpressed CD44 receptor on gastric cancer cells, was clearly identified by the presence of a greyish shell on the dark mesoporous silica nanoparticles. Confocal laser-scanning microscopy and flow cytometry analysis showed higher cellular internalization of HA/RSVmirNP compared to RSVmirNP. In vitro cytotoxicity and apoptosis assays confirmed the superior anticancer effect of the optimized formulation and synergistic effects of anti-miR21 and RSV in gastric cancer cells. Importantly, HA/RSVmirNP showed significant (p < 0.001) reductions in the tumor burden compared to the other group. Indeed, HA/RSVmirNP showed a 3-fold higher tumor regression effect compared to that of free RSV and a 2-fold tumor regression effect compared to that of RSVmirNP, indic...