Tumor-associated B cells and humoral immune response in head and neck squamous cell carcinoma

2019
ABSTRACTB lymphocytes are important players in immune responses to cancer. However, their composition and function in headand neck squamous cell carcinoma(HNSCC) has not been well described. Here, we analyzed B cell subsets in HNSCC (n = 38), non-cancerous mucosa (n = 14) and peripheral blood from HNSCC patients (n = 38) and healthy controls (n = 20) by flow cytometry. Intratumoral B cells contained high percentages of activated ( CD86+), antigen-presenting ( CD86+/CD21−) and memory B cells(IgD−/CD27+). T follicular helper cells (CD4+/ CXCR5+/CD45RA−/CCR7−) as key components of tertiary lymphoid structures and plasma cells made up high percentages of the lymphocyte infiltrate. Percentages of regulatory B cellvaried depending on the regulatory phenotype. Analysis of humoral immune responses against 23 tumor-associated antigens (TAA) showed reactivity against at least one antigen in 56% of HNSCC patients. Reactivity was less frequent in human papillomavirus associated (HPV+) patients and healthy controls c...
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