PEGylated BF2-Azadipyrromethene (NIR-AZA) fluorophores, for intraoperative imaging
2019
Abstract Clinical imaging utilising near-infrared fluorescence is growing as an intraoperative aid for the decision-making processes during complex surgical procedures. Existing uses include perfusion assessment and lymph node identification with many new applications currently being proposed and developed. While imaging hardware and software have significantly progressed in recent times, suitable NIR-
fluorophoresremain a limiting factor. In this report, we describe the design, synthesis, photophysical characterization and in vivo imaging assessment of new
PEGylatedNIR-
fluorophoresbased on the BF 2 -azadipyrromethene
fluorophoreclass. The synthetic route includes
PEGylationas the final step, thereby allowing routine access to derivatives substituted with different molecular weights of PEG. Absorption and emission wavelength maxima in PBS lie at 690 and 720 nm respectively with quantum yields over 12%. They show excellent photostability and no light induced singlet oxygen production. A time-course of NIR-fluorescence imaging, post i.v. administration, in BALB/c mice showed a rapid and preferential accumulation in the renal excretion pathway within 20 min, indicative of potential clinical usage for intraoperative identification of vial structures along this pathway. Assessment with clinical imaging equipment showed the NIR-AZA
fluorophoresto be wavelength compatible and brighter than currently used methylene blue (MB), and that they have the ability to be imaged simultaneously with
indocyanine green(ICG) offering a potential for dual colour clinical imaging.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
44
References
11
Citations
NaN
KQI