Revisiting the genotyping scheme for varicella-zoster viruses based on whole-genome comparisons

We report whole-genome sequences(WGSs) for four varicella-zoster virus(VZV) samples from a shinglesstudy conducted by Kaiser Permanente of Southern California. Comparative genomicsand phylogenetic analysis of all published VZV WGSs revealed that strain KY037798 is in cladeIX, which shall henceforth be designated clade9. Previously published single nucleotide polymorphisms (SNP)-based genotyping schemes fail to discriminate between clades6 and VIII and employ positions that are not clade-specific. We provide an updated list of clade-specific positions that supersedes the list determined at the 2008 VZV nomenclature meeting. Finally, we propose a new targeted genotyping scheme that will discriminate the circulating VZV cladeswith at least a twofold redundancy. Genotyping strategies using a limited setof targeted SNPs will continue to provide an efficient ‘first pass’ method for VZV strain surveillance as vaccination programmes for varicella and zoster influence the dynamics of VZV transmission.