Antiproliferative effects of anastrozole on MCF-7 human breast cancer cells in vitro are significantly enhanced by combined treatment with testosterone undecanoate.

Abstract The present study aimed to assess the effects of aromatase inhibitor anastrozoleand testosterone undecanoate, separately and in combination, on proliferation and apoptosis in MCF-7human breast cancer cells cultured in vitro. The effects of various concentrations of these drugs on the proliferation of MCF-7cells were evaluated by CCK8 assay, the levels of cell apoptosis were evaluated by flow cytometry with Annexin-V/ propidium iodidestaining and androgen receptor (AR) protein expression was determined by western blot analysis. The results of the CCK8 assay indicated that greater antiproliferative activity was detected in the MCF-7cells in the combined treatment groups, compared with those treated with anastrozoleor testosterone undecanoatealone. Flow cytometric analysis of apoptosis revealed that treatment with a combination of the two drugs generated a higher percentage of apoptotic cells, particularly when the two drugs were applied for 48 h, compared with single drug treatment. Western blot analysis revealed a significant decrease in AR protein expression in the combined treatment groups compared with MCF7 cells treated with single drugs. The results of the present study provided evidence supporting the potential of a combination of anastrozoleand testosterone undecanoateas a novel therapeutic strategy for the treatment of breast cancer. Furthermore, it was demonstrated that the antiproliferative effects of anastrozolewere significantly enhanced by combined treatment with testosterone undecanoatevia the AR signaling pathway.