MicroRNA‑543 inhibits proliferation, invasion and induces apoptosis of glioblastoma cells by directly targeting ADAM9
2017
Glioma is the most common type of
malignant brain tumorin humans and accounts for 81% of all
malignant brain tumorcases in adults. The abnormal expression of microRNAs (
miRs) has been reported to be important in the formation and progression of various types of human cancer, including glioblastoma (GBM). Therefore, studies into the expression, and roles of microRNAs as diagnostic and prognostic markers, as well as their therapeutic value for patients with GBM are warranted. The expression and roles of
miR‑543 have been reported in several types of human cancer. However, the role of
miR‑543 in GBM remains unclear. In the current study, the expression pattern of
miR‑543 in GBM, the effects of
miR‑543 on GBM cells and the underlying molecular mechanism was determined. The results of the present study demonstrated that
miR‑543 was significantly downregulated in GBM tissue samples and cell lines. Furthermore, the upregulation of
miR‑543 inhibited GBM cell proliferation and invasion, as well as promoted cell apoptosis. In addition, a
disintegrinand metalloproteinase 9 (
ADAM9) was identified to be a direct target gene of
miR‑543. Furthermore,
ADAM9was significantly upregulated in GBM tissue samples and its expression was inversely correlated with
miR‑543 expression in GBM tissue, suggesting that
miR‑543 downregulation may contribute to
ADAM9upregulation in GBM. Finally, the results of the rescue experiment indicated that
ADAM9overexpression significantly reversed the effects of
miR‑543 on the proliferation, invasion and apoptosis of GBM cells, suggesting that
miR‑543 serves as a tumor suppressor in GBM through
ADAM9regulation. Overall, these findings indicate that the
miR‑543/
ADAM9signaling pathway may provide as a potential therapeutic strategy for GBM.
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