Scientific Research
Zhilong Wang
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Overview
Dr. Zhilong Wang, Professor (researcher) of School of Pharmacy and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Editor of Biotechnology and Bioprocess Engineering.
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Research Field
Biocatalysis, biotransformation, and microbial fermentation are usually limited by the low substrate solubility, the substrate/product inhibition, and the further product degradation. Our interesting is integration of bio-reaction into its downstream extractive process to solve the above problems. Based on a novel medium (cloud point system) developed in our lab, successful examples are carried out, including microbial transformation of water-insoluble of cholesterol to produce intermediate androst-1, 4-diene-3, 17-dione (ADD) of steroid medicine, microbial transformation of toxic benzaldehyde into Chinese traditional medicine L-phenylacetylcarbinol (L-PAC), and extractive fermentation of intracellular Monascus pigments (Chinese traditional colorant and folk medicine) etc.
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Publications & Patents
1. Zhilong Wang, Extractive Microbial Transformation (Book). 2012, Chemical industry Press, Beijing, China
2. Biyu Kang, Xuehong Zhang, Zhenqiang Wu, Hanshi Qi, Zhilong Wang*. Solubilization capacity of nonionic surfactant micelles strong influence on export of intracellular pigments in Monascus fermentation. Microbial biotechnology. 2013, doi:10.1111/1751-7915.12039
3. Biyu Kang, Xuehong Zhang, Zhenqiang Wu, Hanshi Qi, Zhilong Wang*. Effect of pH and nonionic surfactant on profile of intracellular and extracellular Monascus pigments. Process biochemistry, 2013, 48: 759-767
4. Pradip B. Dhamole, Zhilong Wang, Yuanqin Liu, Bin Wang, Hao Feng*. Extractive fermentation with non-ionic surfactants to enhance butanol production. Biomass and bioenergy. 2012, 40: 112-119
5. Zhiqiang Hu, Xuehong Zhang, Zhenqiang Wu*, Hanshi Qi, Zhilong Wang*. Perstraction of intracellular pigments by submerged cultivation of Monascus in nonionic surfactant micelle aqueous solution. Applied microbiology and biotechnology. 2012, 94: 81-89
6. Zhiqiang Hu, Xuehong Zhang, Zhenqiang Wu, Hanshi Qi, Zhilong Wang*. Export of intracellular Monascus pigments by two-stage microbial fermentation in nonionic surfactant micelle aqueous solution. Journal of biotechnology. 2012, 162: 202-209
7. Zhilong Wang*. Bioavailability of organic compound solubilized in nonionic surfactant micelles (review). Applied microbiology and biotechnology. 2011, 89: 523-534
8. Zhilong Wang*, Jian-He Xu, Hao Feng, Hanshi Qi. Fractal kinetic analysis of polymers/nonionic surfactants to eliminate lignin inhibition in enzymatic saccharification of cellulose. Bioresource technology. 2011, 102: 2890-2896
9. Mingjing Yao, Zhilong Wang*, Zhenqiang Wu*, Hanshi Qi. Evaluating kinetics of enzymatic saccharification of lignocellulose by fractal kinetic analysis. Biotechnology and bioprocess engineering. 2011, 16: 1240-1247
10. Yingying Xue, Chen Qian, Zhilong Wang*, Jian-He Xu, Yude Yang, Hanshi Qi. Investigation of extractive microbial transformation in direct nonionic surfactant micelle aqueous solution by response surface methodology. Applied microbiology and biotechnology. 2010, 85(1): 517-524
11. Zhilong Wang*, Rui Liang, Jian-He Xu, Hanshi Qi. A closed concept of extractive whole cell microbial transformation of benzaldehyde into L-phenylacetylcarbinol by Saccharomyces cerevisiae in novel polyethylene-glycol-induced cloud-point system. Applied biochemistry and biotechnology. 2010, 160 (6): 1865-1877
12. Tao Pan, Zhilong Wang*, Jian-He Xu, Zhenqiang Wu*, Hanshi Qi. Extractive fermentation in cloud point system for lipase production by Serratia marcescens ECU1010. Applied microbiology and biotechnology. 2010, 85(6): 1789-1796
13. Tao Pan, Zhilong Wang*, Jian-He Xu, Zhenqiang Wu, Hanshi Qi. Stripping of nonionic surfactant from coacervate phase of cloud point system for lipase separation by Winsor II microemulsion extraction with direct addition of alcohols. Process biochemistry. 2010, 45: 771-776
14. Zhilong Wang*, Zewen Dai. Extractive microbial fermentation in cloud point system (review). Enzyme and microbial technology. 2010, 46: 407-418
15. Zhilong Wang, Hao Feng*. Double cloud point of ethylene oxide-propylene oxide triblock copolymer in an aqueous solution. Colloid and surface A: Physicochem Eng Aspects. 2010, 362: 110-116
16. Zhilong Wang*, Hao Feng*. Fractal kinetic analysis of the enzymatic saccharification of cellulose under different conditions. Bioresource technology. 2010, 101: 7995-8000
17. Zewen Dai, Zhilong Wang*, Jian-He Xu, Hanshi Qi. Assessing bioavailability of the solubilization of organic compound in nonionic surfactant micelles by dose-response analysis. Applied microbiology and biotechnology. 2010, 88: 327-339
18. Rui Liang, Zhilong Wang*, Jian-He Xu, Wei Li, Hanshi Qi. Novel polyethylene glycol induced cloud point system for extraction and back-extraction of organic compounds. Separation and purification technology. 2009, 66: 248-256
19. Zhilong Wang*. Predicting organic compound recovery efficiency of cloud point extraction with its quantitative structure-solubilization relationship. Colloids and surfaces A: Physicochem Eng Aspects. 2009, 349: 214-217
20. Zhilong Wang*, Jian-He Xu, Wenzhi Zhang, Baohua Zhuang, Hanshi Qi. Cloud point of nonionic surfactant Triton X-45 in aqueous solution. Colloids and surfaces B: Biointerfaces. 2008, 61:118-122
21. Zhilong Wang*, Wenzhi Zhang, Jian-He Xu, Baohua Zhuang, Hanshi Qi. In situ extraction of polar product of whole cell microbial transformation with polyethylene glycol induced cloud point system. Biotechnology progress. 2008, 24(5): 1090-1095
22. Wenzhi Zhang, Zhilong Wang*, Wei Li, Baohua Zhuang, Hanshi Qi. Production of L-phenylacetylcarbinol by microbial transformation in polyethylene glycol induced cloud point system. Applied microbiology and biotechnology. 2008, 78: 233-239
23. Zhilong Wang*, Jian-He Xu, Rui Liang, Hanshi Qi. A downstream process with microemulsion extraction for microbial transformation in cloud point system. Biochemical Engineering Journal. 2008, 41:24-29
24. Zhilong Wang*, Jian-He Xu, Daijie Chen. Whole cell microbial transformation in cloud point system. Journal of industrial microbiology and biotechnology. 2008, 35:645-656
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contact information
School of Pharmacy, Shanghai Jiao Tong University
800 Dongchuan Road, Shanghai, 200240
zlwang@sjtu.edu.cn
